I mean obviously you’d use DMSO or glycerol, I just didn’t want to get too technical. That being said I’ve always snap-frozen bacteria with LN2 and it worked just fine.
Now for eucaryotic cells, sure, you’d want to go slow.
Edit : ok now re-reading the meme I understand why you thought I was talking about eucaryotic cells. My bad !
I mean obviously you’d use DMSO or glycerol, I just didn’t want to get too technical. That being said I’ve always snap-frozen bacteria with LN2 and it worked just fine. Now for eucaryotic cells, sure, you’d want to go slow.
Edit : ok now re-reading the meme I understand why you thought I was talking about eucaryotic cells. My bad !